METHOD OF MANUFACTURING REFERENCE MATERIAL USING PLANT CULTURED CELL LINES

摘要

The present invention relates to a method of manufacturing a reference material for detecting genetically modified (GM) plants mixed into samples or for analyzing mixing ratios from tissue-cultured cell lines that are obtained by culturing tissues of either GM plants or non-GM plants. The present invention further relates to a method of detecting and analyzing ratios of GM plants mixed into samples using the reference material. With the reference material of the present invention, which is for detecting intermixing or for analyzing the mixing ratio of GM plants in samples by using tissue-cultured cell lines that are obtained by respectively culturing tissues of either GM plants or non-GM plants, countless individual lines having the same genetic traits can be obtained via the cell culturing, and hence the invention allows culturing capacity to be increased to large volumes in such a way as to produce large volumes of reference material of uniform quality free from quality variation between batches. In addition, reference materials manufactured by using grain powder, the present invention has advantages in that it allows either GM or non-GM reference materials with 100% purity to be obtained by verifying the purity of tissue-cultured cell lines, which enables a constant stable supply of reference materials with uniform compositions.