METHOD FOR IMPROVING PERFORMANCE OF RT-PCR USING KLENOW FRAGMENT

摘要

The present invention relates to a method for improving performance of PT-PCR, and more particularly, to a method for improving sensitivity and amplification efficiency of RT-PCR by adding denatured Klenow fragment to an RT-PCR solution. The method of the present invention reduces DNA polymerase activity inhibition caused by a reverse transcriptase and improves the amplification efficiency of PCR, thereby improving the performance of RT-PCR.