发明名称 |
Method for assaying protein-protein interaction |
摘要 |
The invention relates to a method for determining if a test compound, or a mix of compounds, modulates the interaction between two proteins of interest. The determination is made possible via the use of two recombinant molecules, one of which contains the first protein, a cleavage site for a proteolytic molecule, and an activator of a gene. The second recombinant molecule includes the second protein and the proteolytic molecule. If the test compound binds to the first protein, a reaction is initiated whereby the activator is cleaved, and activates a reporter gene. |
申请公布号 |
EP2336768(A1) |
申请公布日期 |
2011.06.22 |
申请号 |
EP20100186070 |
申请日期 |
2004.07.09 |
申请人 |
LIFE TECHNOLOGIES CORPORATION;THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK |
发明人 |
LEE, KEVIN J.;AXEL, RICHARD;STRAPPS, WALTER;BARNEA, GILAD |
分类号 |
G01N33/50;C12N;C12N15/10;C12N15/85;C12Q1/37;C12Q1/68;G01N33/53;G01N33/542;G01N33/68;G01N33/94 |
主分类号 |
G01N33/50 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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