USE OF MONOOLEIN AS A NEW AUXILIARY LIPID IN LIPOFECTION

摘要

The present invention relates to the application of monoolein-based cationic lipid systems for the complexation, transport and transfection of genetic material into target cells. The lipid systems are prepared through the conjugation of the neutral lipid monooleoyl-rac glycerol (Monoolein) with the cationic tensioactives derived from Dioctadecyldimethylammonium (DODAX) as, for example, Dioctadecyldimethylammonium Bromide (DODAB), Dioctadecyldimethylammonium Chloride (DODAC) and Dioctadecyldimethylammonium Phosphate (DODAF). The lipofection method here describe, presenting high levels of biocompatibility and reduced acquisition costs, is applicable for molecular biology purposes ( in vitro application), as well as in the health area in gene therapy ( i n vivo application).