| 摘要 |
A hybrid formed of a probe nucleic acid immobilized onto a substrate and a sample nucleic acid, in which the target strand of the sample nucleic acid is longer than the probe nucleic acid, so that at least a 5′ end portion of the target strand extends upstream the target sequence; a 3′ end of the target strand is the same as a 3′ end of the target sequence or extends downstream the target sequence by at least one base from the 3′ end of the target sequence; and provided that when the number of bases of the extending 5′ portion is designated as L1, and the number of bases of the extending 3′ portion of the target strand is designated as L2, a value of L1/L2 falls within the range of 0 to 1.5 both inclusive. This hybrid is very stable so that useful for detecting a small amount of nucleic acid specifically with high sensitivity.
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