发明名称 |
Use of rpoB gene for in vitro diagnosis of a pathogenic strain of Leptospira in a biological sample, where the biological sample is blood, urine, cerebrospinal fluid, aqueous humor, serum, and organs such as the kidney |
摘要 |
<p>Use of rpoB gene for in vitro diagnosis of a pathogenic strain of Leptospira, is claimed. Independent claims are included for: (1) in vitro method to detect the presence of at least one pathogenic strain of Leptospirain a biological sample comprising analysis of biological sample for sequence having at least 85% of identity with the sequence of the region 98-237 (SEQ ID NO: 2) of the rpoB gene of Leptospira interrogans serovar Copenhagen, in which the detection of the sequence indicates the presence of at least one pathogenic Leptospirastrain in the sample; and (2) a kit for detecting the presence of at least one pathogenic strain of Leptospirain the biological sample containing at least one oligonucleotide comprising: DNA sequence of SEQ ID NO: 25 (comprising gtcttgcctt ccgagtcg), SEQ ID NO: 26 (comprising tctgcaagagttrttgaccatc) or SEQ ID NO: 27 (comprising cgcacgtccgagcatatcgtcg); DNA sequence complementary to the sequences; RNA sequence corresponding to SEQ ID NO: 25, SEQ ID NO: 26 or SEQ ID NO: 27; and RNA sequence complementary to the sequences.</p> |
申请公布号 |
FR2953858(A1) |
申请公布日期 |
2011.06.17 |
申请号 |
FR20090058895 |
申请日期 |
2009.12.11 |
申请人 |
ECOLE NATIONALE VETERINAIRE DE LYON |
发明人 |
KODJO ANGELI;DJELOUADJI ZOHEIRA |
分类号 |
C12Q1/68;C12Q1/04 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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