发明名称 |
USES FOR LONG POLAR FIMBRIAE GENES OF PATHOGENIC ESCHERICHIA COLI STRAINS |
摘要 |
Provided are methods for identifying lpf genes in pathogenic serotypes of the Enterobacteriaceae family and for differentiating Escherichia coli (E. coli) O157:H7 strains in an isolate using primer pairs specific to lpf gene variants, particularly IpfA1 and/ or lpfA 2 genes and amplicon size of the PCR product to identify prototypic Enterobacteriaceae serotypes or more specifically, to differentiate strains of E. coli serotype O157:1-17. Differentiation further requires identifying the E. coli isolate's eae gene variant type which in combination with the IpfA1 and/or lpfA2 variant identification provides unique markers. Also provided are the primer pairs and a kit comprising the same. |
申请公布号 |
WO2010117465(A3) |
申请公布日期 |
2011.06.16 |
申请号 |
WO2010US01065 |
申请日期 |
2010.04.09 |
申请人 |
THE BOARD OF REGENTS OF THE UNIVERSITY OF TEXAS SYSTEM;TORRES, ALFREDO, G. |
发明人 |
TORRES, ALFREDO, G. |
分类号 |
C12Q1/68;C12N15/31;C12R1/19 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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