| 摘要 |
PROBLEM TO BE SOLVED: To provide a confocal scanning microscope capable of suppressing the degradation of the quality of a fluorescent image caused by self-fluorescence, while allowing a confocal diaphragm to act on exciting light and fluorescence. SOLUTION: The confocal scanning microscope 1 guides the exciting light emitted from a light source part 2 to an objective optical system 6 by a light separation means 3 and irradiates a sample 12 arranged in the focal position on the side of the sample 12 of the objective optical system 6. The confocal scanning microscope 1 makes the fluorescence generated from the sample 12 incident on the light separation means 3 via the objective optical system 6, to separate the fluorescence from the exciting light and detects the fluorescence by an imaging element 19 via an imaging optical system 13. The confocal scanning microscope 1 produces a confocal effect by a line slit member 5a arranged in a position optically conjugate to the focal position on the side of the sample 12 of the objective optical system 6, between the light source part 2 and the objective optical system 6 and intercepts the self-fluorescence by a line slit member 15a arranged in a position optically conjugate to the line slit member 5a in the imaging optical system 13. COPYRIGHT: (C)2011,JPO&INPIT
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