IMPROVED METHODS OF CELL CULTURE FOR ADOPTIVE CELL THERAPY
摘要
An improved method of culturing cells for cell therapy applications that includes growing desired cells in the presence of antigen-presenting cells and/or feeder cells and with medium volume to surface area ratio of up to 1 ml/cm2 if the growth surface is not comprised of gas permeable material and up to 2 ml/cm2 if the growth surface is comprised of gas permeable material. The desired cells are at a surface density of less than 0.5x106 cells/cm2 at the onset of a production cycle, and the surface density of the desired cells plus the surface density of the antigen presenting cells and/or feeder cells are at least about 1.25 xlO5 cells/cm2.
申请公布号
CA2783550(A1)
申请公布日期
2011.06.16
申请号
CA20102783550
申请日期
2010.12.08
申请人
WILSON WOLF MANUFACTURING CORPORATION
发明人
VERA, JUAN F.;ROONEY, CLIO M.;LEEN, ANN M.;WILSON, JOHN R.