发明名称 |
THERMOSTABLE POLYMERASES HAVING ALTERED FIDELITY AND METHODS OF IDENTIFYING AND USING SAME |
摘要 |
The present invention provides a method for identifying a thermostable polymerase having altered fidelity. The method consists of generating a random population of polymerase mutants by mutating at least one amino acid residue of a thermostable polymerase and screening the population for one or more active polymerase mutants by genetic selection. For example, the invention provides a method for identifying a thermostable polymerase having altered fidelity by mutating at least one amino acid residue in an active site O-helix of a thermostable polymerase. The invention also provides thermostable polymerases and nucleic acids encoding thermostable polymerases having altered fidelity, for example, high fidelity polymerases and low fidelity polymerases. The invention additionally provides a method for identifying one or more mutations in a gene by amplifying the gene with a high fidelity polymerase. The invention further provides a method for accurately copying repetitive nucleotide sequences using a high fidelity polymerase mutant. The invention also provides a method for diagnosing a genetic disease using a high fidelity polymerase mutant. The invention further provides a method for randomly mutagenizing a gene by amplifying the gene using a low fidelity polymerase mutant. |
申请公布号 |
US2011143356(A1) |
申请公布日期 |
2011.06.16 |
申请号 |
US20100939798 |
申请日期 |
2010.11.04 |
申请人 |
UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION |
发明人 |
LOEB LAWRENCE A.;HOOD LEROY;SUZUKI MOTOSHI |
分类号 |
C12Q1/68;C12N9/10;C12N9/12;C12N9/22;C12P19/34;C12Q1/34 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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