METHOD OF QUANTITATIVE ANALYSIS OF CELL NUMBER OF ALIVE BACTERIUM OF INTEREST WITH APPLYING rRNA AS TARGET

摘要

FIELD: medicine. ^ SUBSTANCE: offered invention can be used for the quantitative determination of alive bacteria. The offered method involves PCR-based product amplification with using the primers able to specific hybridisation with rRNA of a bacterium of interest. It is followed with the analysis of cell number of the alive bacterium of interest taking into account PCR cycle number. ^ EFFECT: invention allows precise determination of cell number of the alive bacterium of interest in a test sample. ^ 8 cl, 7 dwg, 4 tbl, 10 ex