| 摘要 |
FIELD: medicine. ^ SUBSTANCE: bacteria freely immobilised on a slide are processed with a lysis solution containing an ionogenic protein-denaturing detergent and EDTA. Further, DNA-nucleoid is dry heating and microwave incubation stabilised. DNA is stained by high-sensitivity DNA-specific fluorochrome, in conclusion DNA integrity is evaluated. Offered is a kit for evaluating the DNA bacteria integrity which comprises all the components required. ^ EFFECT: invention can be used for determining the bacterial DNA fragmentation levels by a precise express technique. ^ 16 cl, 11 dwg, 5 tbl, 9 ex |
