| 摘要 |
The invention relates to methods and compositions for assessing protein stability, including improved assays for distinguishing between soluble and aggregated proteins. The methods and compositions include measuring residual fluorescence of a fusion protein in a soluble fraction as an indicator of protein solubility, and monitoring fluorescence quenching of a fusion protein as an indicator of protein stability. The fusion protein may comprise an amino acid sequence of a protein of interest, a peptide linker amino acid sequence and an amino acid sequence of a fluorescent marker protein.
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