| 摘要 |
<p>The invention relates to medicine, biology, pharmacology, in particular to a method for immobilizing a biologically active substance on a pharmacologically acceptable polymer carrier. The inventive method involves dissolving a biologically active substance in a polymer carrier aqueous solution, the concentration of which is greater than 0%, up to a saturated solution concentration and irradiating the reaction mixture by ionizing radiation. In order to protect the biologically active substance from being destroyed by ionizing radiation, the reaction mixture can be frozen at a temperature ranging from -20°C to -140°C prior to be irradiated. While being immobilized according to the inventive method, the biologically active substance is covalently linked to the polymer. The advantage of the inventive method consists in that it makes possible to immobilize on the polymer carrier those water-soluble biologically active substances which are unstable, i.e., which are destroyed totally or in part when immobilized by the other methods and to increase the portion of the preserved biologically active substance, when the biologically active substance is not totally destroyed during immobilization. The conjugate as a biologically active substance carrier is characterized in that the biologically active substance is selected from a group comprising proinsulin, tyrosyl-2-alanyl-glyceryl-phenylalanyl-leucyl-arginine diacetate, alcalase, hyaluronidase, somatotropin and in that the carrier is in the form of a pharmacologically acceptable water-soluble polymer which is selected from a group comprising polyethyleneoxide, polyvinylpirrolidone, dextran, pluronic and acrilamide, wherein a biologically active substance molecule is covalently linked to at least one polymer molecule.</p> |
