| 摘要 |
In one aspect, the present invention provides methods for producing mature pine somatic embryos. The methods of the invention comprise the steps of: (a) culturing pine embryonal suspensor mass in a maintenance medium to form pre-cotyledonary pine somatic embryos; (b) culturing the pre-cotyledonary pine somatic embryos formed in step (a) in, or on, a development medium comprising maltose as the principal source of metabolizable sugar and less than 0.5% of gellan gum to form cotyledonary pine somatic embryos; and (c) culturing the cotyledonary pine somatic embryos formed in step (b) in, or on, a maturation medium comprising from about 1% to about 6% sucrose to produce mature pine somatic embryos. In some embodiments, the maturation medium further comprises sucrose and /or abscisic acid.
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