| 摘要 |
<p>Disclosed is a primer set for specifically amplifying a target region in MTHFR gene by a nucleic acid amplification method. A primer set, which comprises a primer set (1) that contains an F1 primer and an R1 primer and a primer set (2) that contains an F2 primer and an R2 primer, is used. For example, the primer set makes it possible to amplify two target regions simultaneously in a single reaction mixture, said target regions respectively containing sites at which two kinds of polymorphism of MTHFR gene occur. (F1) an oligonucleotide having a length of 20-28 nucleotides, in which guanine (g) at the nucleotide position 8715 forms the 3' terminus in the base sequence set forth in SEQ ID NO: 1 (R1) an oligonucleotide that is complementary to an oligonucleotide having a length of 18-26 nucleotides, in which cytosine (c) at the nucleotide position 8817 forms the 5' terminus in the base sequence set forth in SEQ ID NO: 1 (F2) an oligonucleotide having a length of 26-36 nucleotides, in which guanine (g) at the nucleotide position 10590 forms the 3' terminus in the base sequence set forth in SEQ ID NO: 1 (R2) an oligonucleotide that is complementary to an oligonucleotide having a length of 22-34 nucleotides, in which cytosine (c) at the nucleotide position 10695 forms the 5' terminus in the base sequence set forth in SEQ ID NO: 1</p> |
