HIGH-AFFINITY TCR PROTEIN AND METHOD

摘要

PROBLEM TO BE SOLVED: To provide a T cell receptor (TCR) that has higher affinity for a ligand than wild type TCR.

SOLUTION: These high-affinity TCR is obtained by steps of: mutagenizing a T cell receptor protein coding sequence to generate a variegated population of mutants of the T cell receptor protein coding sequence; transforming the T cell receptor mutant coding sequence into yeast cells; inducing expression of the T cell receptor mutant coding sequence on the surface of yeast cells; and selecting those cells expressing T cell receptor mutants that have higher affinity for the peptide/MHC ligand than the wild type T cell receptor protein. The high-affinity TCR can be used in place of an antibody or single chain antibody.

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