| 摘要 |
A TIRF illumination having a high axial resolution at low complexity. A TIRF illumination device is designed as a module and comprises an optical fiber and a collimating optic, wherein the collimating optic is mounted in front of a light discharge opening of the optical fiber, such that it collimates light exiting divergently from the optical fiber into a parallel light bundle, such that the excitation light can be applied to a sample outside of the detection beam path. The numerical aperture of the excitation is thus decoupled from the numerical aperture of detection, such that a standard microscope objective is sufficient for detection.
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