发明名称 |
METHODS FOR ENHANCED PROTEIN PRODUCTION |
摘要 |
The present invention provides a method of increasing protein production in a cell culture by growing cells that produce the protein (e.g., the growth phase) in a perfusion cell culture to a high cell density (i.e., at least above about 40x106 cells/mL) and then switching to a protein production phase, wherein the cells are cultured in a fed-batch cell culture. The present invention further provides a method for clarifying a protein from a cell culture by adjusting the pH of the cell culture to below neutral pH (i.e., below a pH of 7) and settling the cell culture, such that the cell culture separates to form a supernatant layer and a cell-bed layer, wherein the protein is in the supernatant layer. |
申请公布号 |
WO2011062926(A2) |
申请公布日期 |
2011.05.26 |
申请号 |
WO2010US56924 |
申请日期 |
2010.11.17 |
申请人 |
MEDAREX, INC.;ARUNAKUMARI, ALAHARI;DAI, XIAO-PING;GARCIA, JAVIER;MARTEL, RICHARD |
发明人 |
ARUNAKUMARI, ALAHARI;DAI, XIAO-PING;GARCIA, JAVIER;MARTEL, RICHARD |
分类号 |
C12P21/00;C07K1/36;C12N1/02;C12N5/00;C12P21/02 |
主分类号 |
C12P21/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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