| 摘要 |
<p>A method increases the signal strength generated when performing real-time PCR on a target nucleic acid sequence. The method performs real-time PCR using forward primers, forward probes, reverse primers, reverse probes, nucleotides for strand/antistrand extension, and nucleic acid polymerase. Two different types of probes are used, a forward probe configured to anneal to a sense strand of a target nucleic acid sequence and a reverse probe configured to anneal to an antisense strand of the target nucleic acid sequence. The forward probe is complementary to an inner sequence of the target sense strand, and the reverse probe is complementary to an inner sequence of the target antisense strand. The forward probe and the reverse probe each include the same detectable element, that when released from the probe during strand extension results in an additive detectable signal.</p> |
