Process for separating single-stranded from double-stranded nucleic acids and the separation column for carrying out this process

摘要

A process insulates and separates single and twin-strand nucleic acids from a sample substrate substance. The twin-stranded nucleic acids are eluted with a first elution solution and the single-stranded nucleic acids are then eluted by a second elution solution. Elution of the twin-stranded nucleic acid takes place in the presence of a bivalent metal ion. The concentration of the bivalent ion is so high, that no single-stranded nucleic acid is eluted. The bivalent eluate solution concentration is at least 1 mM. After binding the nucleic acids the substrate material is washed in a solution containing bivalent metal ions prior to the elution process. The washing solution is an aliphatic alcohol with 1 to 5 carbon atoms and is a buffer solution of pH 4.5 to 9.5. The pH value is set to 7 and the concentration of twin-stranded nucleic acids is no more than 200 mM. An independent claim is also given for a suitable separation column for the process. The column height is such that no single-stranded nucleic acid is eluted.