| 摘要 |
A new antibiotic substance named thermorubin is of weak acidic character and insoluble in water, ethyl ether and petroleum ether, very poorly soluble in methanol and low molecular weight alcohols, ethyl acetate and higher esters, benzene, chloroform and acetone, and very soluble in dioxane, tetrahydrofuran, dimethyl-formamide and pyridine, decomposed at 190 DEG C. (darkening) and has a melting point not lower than 300 DEG C., its elemental analysis being C=64.15%, H=4.24%, 0=31.50% and approximate molecular weight determined cryoscopically of 432 indicating the approximate formula C22H18O8; thermorubin forms salts with alkalies, has specific rotatory power [a ]25D = + 14 DEG (C = 0.4 in dioxane), shows ultra-violet absorption maxima at 300, 328, 414 and 430 mm and details are given in the Specification of absorption bands in the infra-red. Thermorubin is produced by cultivating under aerobic conditions a thermorubin producing strain of Thermoactinomyces antibioticus, particularly T. antibioticus ATCC 14570, in aqueous medium containing a source of carbon, nitrogen and essential inorganic salts, preferably for 12 to 48 hours at about 50 DEG C. and pH 6.5-10.5, and recovering the antibiotic from the medium, preferably by solvent extraction.
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