| 摘要 |
<p><P>PROBLEM TO BE SOLVED: To provide a method for determining the presence of immobilized nucleic acid, by employing: unsymmetrical cyanine dyes that are derivatives of thiazole orange; a staining solution; and carefully-selected fluorogenic compounds that are characterized as being essentially non-genotoxic. <P>SOLUTION: The method includes processes of: immobilizing nucleic acid, single or double stranded DNA, RNA or a combination thereof, on a solid or semi-solid support; contacting the immobilized nucleic acid with an unsymmetrical cyanine dye compound; and then illuminating the immobilized nucleic acid with an appropriate wavelength whereby the presence of the nucleic acid is determined. <P>COPYRIGHT: (C)2011,JPO&INPIT</p> |
