INDIRECT ELISA KIT USING HEPARIN-TREATED PRRSV AND MANUFACTURING METHOD THEREOF

摘要

PURPOSE: A method for manufacturing an ELISA kit using heparin-treated PRRSV antigen is provided to minimize non-specific reaction and to significantly detect a target antibody of PRRSV. CONSTITUTION: A method for manufacturing ELISA kit for detecting PRRSV antibody comprises: a step of mixing PRRSV antigen and heparin; and a step of coating the surface of a dish using the mixture. The PRRSV antigen is CP07-401-9, LCS-EU, or mixture of CP07-401-9 and LCS-EU. A method for diagnosing PRRSV comprises: a step of reacting porcine serum with a dish coated with heparin-treated PRRSV antigen and a dish coated with a control group; a step of reacting with a second antibody; and a step of calculating PRRSV titer.