Extension of the dynamic detection range of assay devices

摘要

A flow-through assay device for detecting the presence or quantity of an analyte residing in a test sample is provided. The device utilizes multiple detection zones, one of which is premised on“competitive”binding of the analyte and the other is premised on“sandwich”binding of the analyte. The present inventors believe that the combination of these zones may enable the detection of an analyte over extended concentration ranges.