| 摘要 |
The present invention relates to a method for producing a miniaturized separation column for chromatography purposes, having a porous stationary phase anchored in the column, comprising the following steps: (a) providing a planar substrate made of silicon, glass, glass ceramic material or ceramic material, (b) etching at least one channel structure into the planar substrate, (c) introducing a non-porous precursor material for the porous stationary phase into at least part of the channel structure(s), (d) forming a porous three-dimensional network from the precursor material, (e) covering the channel structure(s) in a liquid-tight manner on the upper face of the planar substrate. The invention further relates to a miniaturized separation column for chromatography purposes, the lateral walls of which are formed by one or more recesses extending in the surface of a planar silicon, glass, glass ceramic or ceramic substrate and one or more covers of said recess(es), and which is filled with a porous stationary chemical or physical phase forming a three-dimensional network and being joined to at least a portion of the column walls, said phase consisting of an inorganic or an organically modified inorganic or an organic material, selected from the group consisting of metals, ceramics, optionally organically modified oxides and organic polymers, to a wafer having a plurality of miniaturized separation channels, from which said separation channels can be produced by division, and to a chip, which in addition to one or more separation columns contains elements that may be required for liquid chromatography. |
