The present invention provides methods and kits for determining which microRNAs bind to a target mRNA where the methods comprise the steps of (a) creating a bait sequence from the target mRNA, where the bait sequence comprises a label that binds to a binding agent; (b) adding a mixture of microRNAs to the bait sequence; (c) separating the microRNAs that bind to the bait sequence from those microRNAs that do not bind; and (d) identifying the microRNAs that bind to the bait sequence, wherein the microRNAs identified are those that bind to the target mRNA.
申请公布号
WO2011056186(A1)
申请公布日期
2011.05.12
申请号
WO2010US02771
申请日期
2010.10.18
申请人
ALBERT EINSTEIN COLLEGE OF MEDICINE OF YESHIVA UNIVERSITY;SPIVACK, SIMON, D.;SHI, MIAO;HAN, WEIGUO