| 摘要 |
Candida tropicaliscell is new, where the cell (in comparison to its wild type) exhibits a reduced activity of at least one enzyme that is encoded by an intron-free nucleic acid sequence including SEQ ID No. 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65 or 67 or a sequence that is at least 80% identical to the above sequences, where the above sequences are not defined. Independent claims are included for: (1) the preparation of the above cell; and (2) preparing omega -hydroxy carboxylic acid or its ester, where the chain length of carboxylic acid is 6-24C and chain length of alcohol component of the ester is 1-4C (preferably 12-hydroxy dodecanoic acid or its methyl ester) comprising (a) contacting the above cell with a medium containing carboxylic acid or its ester, (b) culturing the cells under conditions such that the carboxylic acid or its esters form the corresponding omega -hydroxy carboxylic acid or its ester and (c) optionally isolating the formed omega -hydroxy carboxylic acid or its ester. |
