发明名称 |
METHOD FOR SCREENING OF QUORUM SENSING INHIBITORS |
摘要 |
PURPOSE: A method for screening a quorum sensing inhibitor using a strain in which a vector is introduced is provided to easily analyze host cell growth with respect to antibiotics. CONSTITUTION: A vector for screening a quorum sensing inhibitor comprises: a gene encoding transcription protein which binds to acyl homoserine lactone; a promoter and a gene encoding a protein which suppresses antibiotic lyase inhibitor protein which is operatively lined to the promoter; and a gene encoding antiobic lyase. The antibiotic lyase inhibitor protein is beta-lactamase inhibitor protein(BLIP). A method for screening the quorum sensing inhibitor comprises: a step of determining the concentration of acyl homoserine lactone and antibiotics; a step of contacting the strain with quorum sensing inhibitor candidate; and a step of determining quorum sensing inhibitor.
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申请公布号 |
KR20110049193(A) |
申请公布日期 |
2011.05.12 |
申请号 |
KR20090106098 |
申请日期 |
2009.11.04 |
申请人 |
KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY |
发明人 |
KIM, HAK SUNG;KIM, JIN HYUN;LEE, SANG CHUL;JEONG, YOUNG SU |
分类号 |
C12N15/63;C12N15/52;G01N33/15 |
主分类号 |
C12N15/63 |
代理机构 |
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地址 |
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