| 摘要 |
<p><P>PROBLEM TO BE SOLVED: To provide a method for measuring a bicarbonate concentration and a solution (reagent) used for the same in which a measuring performance is not deteriorated even while the reagent for measurements remains exposed to an atmosphere for a long time. <P>SOLUTION: The method for measuring bicarbonate concentration including the steps of: (A) reacting bicarbonate ions and phosphoenolpyruvic acid in a reagent under phosphoenolpyruvic acid carboxylase presence to produce oxaloacetic acid; (B) reacting the oxaloacetic acid produced in the step (A) and NADH or its analog under maleic acid dehydrogenase presence; (C) and measuring a decrease of NADH or its analog, wherein the step (A) is performed in a solution of pH7 or more and less than pH8, the ratio of a front area of the solution in contact with the atmosphere to a volume of the solution is less than 0.2, and phosphoenolpyruvic acid carboxylase is acetic acid bacteria based phosphoenolpyruvic acid carboxylase. <P>COPYRIGHT: (C)2011,JPO&INPIT</p> |
