| 摘要 |
<p><P>PROBLEM TO BE SOLVED: To provide a method wherein the cholesterol in the HDL subfractions of ApoE-containing HDL-C and ApoE-deficient HDL-C, or in all HDL-C can be either separately or simultaneously quantified. <P>SOLUTION: A surfactant selected from the following group is added to a test sample: a surfactant having an ApoE-containing HDL reaction rate/ApoE-deficient HDL reaction rate ratio which is at least 0.7 but less than 1.3, a surfactant having an ApoE-containing HDL reaction rate/ApoE-deficient HDL reaction rate ratio of less than 0.7, and a surfactant having an ApoE-containing HDL reaction rate/ApoE-deficient HDL reaction rate ratio of 1.3 or higher. Cholesterol esterase and cholesterol oxidase are allowed to act upon the resulting test sample, and by quantifying the hydrogen peroxide generated, the cholesterol in the ApoE-containing HDL and the cholesterol in the ApoE-deficient HDL are fractionally quantified using enzymes. <P>COPYRIGHT: (C)2011,JPO&INPIT</p> |
