Glycogen synthase 1 Gene and Recombinant Vector Comprising the Gene and Host Cell Transformed with the Vector and Manipulation of Fatty Acid Composition and Contents via Glycogen Synthase 1-Defective Cyanobacterium Synechocystis PCC 6803

摘要

PURPOSE: A gene gIgA1 encoding glycogen synthase 1 from cyanobacteria is provided to control total content of cyanobacteria by controlling biosynthesis. CONSTITUTION: A gIgA1 gene contains a base sequence of sequence number 1. A recombinant vector contains the gIgA1 gene relating to glycogen biosynthesis of cyanobateria. A host cell which is transformed by the recombinant vector is cyanobacteria PCC6803. A method for preparing transformed cyanobacteria comprises: a step of extracting chromosomal DNA from cyanobacteria Synechocystis PCC6803 wild type; a step of performing PCT using upstream and downstream of the isolated gIgA1 gene a primer; a step of performing PCR of the PCR product and T vector as a template together with antibiotic reactant; a step of cloning mutant cassette to pTOP V2 blunt vector (Enzynomics, Korea); and a step of transforming Synechocystis extract.