A recombinant vector introduced with gene which effectively degrade cellulose, a transformant comprising the vector and a method for producing ethanol using the same

摘要

PURPOSE: A yeast transformant containing a chimera endoglucanase gene and small cellulose-binding protein gene is provided to develop feed and probiotics. CONSTITUTION: A recombinant vector, pADHcCelEmCbpA contains: a chimera gene in which dockerin module is linked to a Clostridium sp.-derived endoglucanase active site gene; and mCbpA gene which is a part of cellulose-binding protein-A derived from Clostridium sp. The endoglucanase active site contains an amino acid sequence of sequence number 1.