发明名称 |
RECOMBINANT TYPE II RESTRICTION ENDONUCLEASES, MmeI AND RELATED ENDONUCLEASES AND METHODS FOR PRODUCTING THE SAME |
摘要 |
In accordance with the present invention, there is provided a DNA (deoxyribonucleic acid) fragment which encodes the MmeI type II restriction endonuclease enzyme. This one polypeptide possesses two related enzymatic functions; namely an endonuclease activity which recognizes the DNA sequence 5'-TCC(Pu)AC-3' and cleaves as indicated by the arrows: 5 ' -TCCRAC (N20) †“-3 ' 3'-AGGYTG(N18)†‘-5' and a second enzymatic activity that recognizes the same DNA sequence, 5'-TCC(Pu)AC-3', but modifies this sequence by the addition of a methyl group to prevent cleavage by the MmeI endonuclease activity. |
申请公布号 |
PL208400(B1) |
申请公布日期 |
2011.04.29 |
申请号 |
PL20030374263 |
申请日期 |
2003.07.10 |
申请人 |
NEW ENGLAND BIOLABS, INC. |
发明人 |
MORGAN RICHARD D.;BHATIA TANYA;DAVIS THEODORE;LOVASCO LINDSAY |
分类号 |
C12N15/52;C12N1/21;C12N9/22;C12N15/00;C12N15/09;C12N15/66 |
主分类号 |
C12N15/52 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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