| 摘要 |
A concept of high resolution electrophoretic technique, apparatus and its application thereof. The system is a functioning entity in a radial-flat setting. Unlike the conventional rectangular gel-electrophoresis setup, wherein two electrically opposite poles are placed on opposite sites of a rectangular gel, the novel system places one of the electrical poles in the middle of the gel and the outer rims of the radial gel is exposed to the other pole. The point, central pole (positive/negative) and the radial (negative/positive) pole around the outer rim of the gel will create and maintain a gradient electric field, wherein the intensity of the electric field increases towards the central pole with an inverse proportionally to the decrease in the surface area towards the center in a radial-gradient setting. When combined with the gradient gel setup, which increases towards the center of the gel in agarose, PAGE, and in any-other media as well as gradient and non-gradient and any-other means the system has a higher resolution potential. At higher voltages moderate heating of the central electrode forms a natural heat gradient, suitable for SSCP and DGGE analyses. Additionally, radial design bestows the system better data visualization and recording potential comparable to DVD writing and reading technology. |
