NOVEL PROCESS

摘要

The present invention relates to a process for detaching cells of an adherent cell type from particles of carrier which comprises the steps of: (a) providing in a vessel said cells adhered to particles of carrier in the presence of a growth medium, said vessel being characterised in that it comprises two internal zones, A and B, separated by a sieve, the sieve having an aperture size such that it is pervious to the cells if detached from the particles of carrier but substantially impervious to the particles of carrier, whereby the particles of carrier having cells adhered thereto are located within zone A but not zone B and zone B contains a drain opening under control of a valve adapted to permit vacation of liquid from zone A under an influence comprising gravity and/or pressure; (b) optionally washing the particles of carrier having cells adhered thereto by vacating liquid from the vessel via the drain opening in zone B under an influence comprising gravity and/or pressure and replenishing the vessel with another wash liquid and optionally repeating this step one or more times; (c) replacing the liquid in the vessel with a detachment buffer by vacating liquid from the vessel via the drain opening in zone B under an influence comprising gravity and/or pressure and replenishing the vessel with the detachment buffer; (c) detaching the cells from the particles of carrier by a process comprising an enzymatic detachment step performed in a detachment buffer.