发明名称 |
RECOMBINANT EXPRESSION VECTOR FOR ANIMAL CELL |
摘要 |
<p>The present invention relates to a recombinant expression vector for an animal cell containing a dihydrofolate reductase (DHFR) coding nucleotide sequence operatively linked to a DHFR promoter, to an animal cell line transformed by the vector, and to a method for preparing a target protein using the same. As compared with existing animal cell expression vectors, the vector of the present invention enables an effective screening of a cell line clone in which foreign genes are amplified together with DHFR genes even at a much lower methotrexate concentration. The present invention exhibits excellent effects in cell line preparation as high-productivity cell lines can be ensured in a short time through the use of a lower concentration of methotrexate in the process of protein production cell line establishment.</p> |
申请公布号 |
EP2314701(A2) |
申请公布日期 |
2011.04.27 |
申请号 |
EP20090794633 |
申请日期 |
2009.07.07 |
申请人 |
APROGEN, INC. |
发明人 |
KIM, KEUN SOO;JU, HYE KYUNG;JEON, CHOON JU;KIM, JAE SUP |
分类号 |
C12N15/64;C12N5/10;C12N9/06;C12N15/85 |
主分类号 |
C12N15/64 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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