METHOD FOR ASSESSING CLINICAL EFFICACY OF ANTIBACTERIAL PREPARATIONS FOR SPECIAL DANGER INFECTIOUS AGENTS Francisella tularensis AND Brucella spp

摘要

FIELD: medicine. ^ SUBSTANCE: assessment of the clinical efficacy of antibacterial preparations is three-staged; the first stage involves working up an antibiotic-sensitivity criteria scale for reference strains; at the second stage the antibiotic-sensitivity of an investigated material is evaluated, while the third stage consists in correlating the latter values with the derived scale for a given type of microbe; the first and second stages are simultaneous and based on the same technique by inoculation of a microbial suspension in the amount 0.3 ml (n107CFU/ml) by a diffusion test and a dilution test on a nutrient medium with growth supplements. Thereafter, the inoculation is incubated at 37C for 24-48 hours, and the results are analysed. The assessment of the clinical efficacy is enabled by correlating the border values of minimal inhibitory concentration and growth inhibition zone diametres with the relevant criteria scale. If the values of the investigated material fall within a permissible range of the criteria scale, an antibiotic is considered to be effective. ^ EFFECT: use of the method enables the reliable and immediate assessment of the clinical efficacy of antibacterial preparations for tularemic and brucellous infections. ^ 7 tbl, 3 ex