发明名称 |
Double brilliance beta-arrestin: a biosensor for monitoring the activity of receptors and signalling molecules, and method of using same |
摘要 |
An intramolecular bioluminescence resonance energy transfer (BRET), biosensor for monitoring receptor activity and signalling cascades is disclosed. The “double-brilliance” biosensor sandwiches &bgr;-arrestin (&bgr;-arr) between Renilla luciferase (Luc) and the yellow fluorescent protein (YFP). &bgr;-arr associates with G-protein coupled receptors GPCR following receptor activation, bringing Luc and YPF into close proximity that favours energy transfer. In addition to providing new insights into the agonist-induced conformational rearrangements of &bgr;-arr in living cells, the double-brilliance &bgr;-arr offers a universal biosensor for GPCR activation, allowing the study of native receptors in large-scale screening analysis. The activity of other signalling molecules known to interact with &bgr; arrestin could also be monitored by double brilliance &bgr; arr.
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申请公布号 |
US7932080(B2) |
申请公布日期 |
2011.04.26 |
申请号 |
US20050579482 |
申请日期 |
2005.05.04 |
申请人 |
VALORISATION-RECHERCHE, LIMITED PARTNERSHIP |
发明人 |
BOUVIER MICHEL;CHAREST PASCALE |
分类号 |
C12M1/34;C07K14/435;C07K19/00;C12N5/10;C12N9/02;C12Q1/48;C12Q1/66;G01N21/64;G01N33/566;G01N33/567;G01N33/58;G01N33/76 |
主分类号 |
C12M1/34 |
代理机构 |
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地址 |
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