| 摘要 |
An electrokinetic microfluidic flow cytometer apparatus can include a substrate, a pair of signal and noise detection channels, and a particle detection circuit. The substrate includes an input port, an output port, and a microchannel that fluidly connects the input port and the output port to allow fluid to flow therebetween. The signal and noise detection channels are defined in the substrate and are fluidly connected to the microchannel from locations that are adjacent to each other. The signal and noise detection channels extend in opposite directions away from the microchannel to receive ambient electrical noise. The particle detection circuit is electrically connected to the signal and noise detection channels and generates a particle detection signal in response to a differential voltage across the signal and noise detection channels, which tracks changes in resistivity across an adjacent portion of the microchannel as particles within the fluid move responsive to an electric field along that portion of the microchannel, while at least substantially canceling a common component of the ambient electrical noise received by the signal and noise detection channels. A particle counting circuit counts a number of particles that move through the sensing gate in response to pulses in the particle detection signal.
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