| 摘要 |
A technique for analyzing proteomics data (such as tandem mass-spectrometry data) corresponding to peptides in a sample is described. In a high-speed, low-sensitivity first pass of this analysis technique, analysis parameters, such as the presence of one or more potential modifications to the one or more peptides, are determined using a representative subset of a database of known proteins. For example, a given potential modification in the one or more potential modifications may be determined by comparing matches between measured mass-spectrometry spectra and generated theoretical mass-spectrometry spectra without the given potential modification to matches between the measured mass-spectrometry spectra and generated theoretical mass-spectrometry spectra with the given potential modification. Then, in a lower-speed, higher-sensitivity second pass of the analysis technique, one or more peptides and/or proteins in the proteomics data are identified using the database of known proteins and the determined analysis parameters.
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