ENZYME METHOD OF EXTRACTING OILS AND PROTEINS FROM VEGETABLE MATTER IN AN AQUEOUS MEDIUM

摘要

An enzyme method of extracting oils, proteins and fermentable sugars from vegetable matter in an aqueous medium, including the following steps: a) adding water to the vegetable matter having a suitable particle size; b) adding an enzyme mixture containing at least one cellulase, at least one hemicellulase and at least one pectinase, the ratio between the pectinase activity and the cellulase activity being at least 0.14, the ratio between the pectinase activity and the hemicellulase activity being at least 7.10-3, and the pectinase activity being less than 120 µmol/min/ml; c) incubating the vegetable matter and the enzyme mixture under agitation; d) separation of the reaction medium in order to obtain free oil, an aqueous phase containing proteins and fermentable sugars, and a solid phase; e) optionally separating an emulsion of the free oil or the aqueous phase, and recycling the emulsion in the reaction medium; and f) separating the proteins and fermentable sugars from the aqueous phase.