| 摘要 |
Provided is a laser scanning microscope that allows stable examination by eliminating the influence of ambient light while inhibiting damage to a specimen and fading of fluorescence. Employed is a laser scanning microscope 1 including a culture vessel 6 accommodating a specimen A and capable of maintaining an interior temperature and humidity thereof and an optical system space 5 adjacent to the culture vessel 6 and optically connected to the culture vessel 6. The optical system space 5 includes a scanner 22 that two-dimensionally scans ultrashort pulsed laser light across the specimen A; an objective lens 15 that focuses the scanned ultrashort pulsed laser light on the specimen A and collects light coming from the specimen A; a dichroic mirror 53, disposed between the scanner 22 and the objective lens 15, that splits off the light coming from the specimen A from the laser light; a photodetector 55 that detects the split-off light coming from the specimen A; and a outer cover 2, provided so as to surround the optical system space 5, that blocks light coming from outside the optical system space 5.
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