MASS SPECTROMETRY QUANTITATION OF P450 PROTEIN ISOFORMS IN HEPATOCYTES

摘要

A method for screening a drug for cytochrome P45O (CYP) induction is provided and can include incubating the drug with a microsome-containing biological sample and then quantitating at least one cytochrome P45G isoform. The isofoms can be selected from 2B6, 3A4, ] A2, and 3A5 isoforms. In some embodiments, the method uses liquid chromatography tandem mass spectrometry (LC-MSMS). A quantitated value can be compared to a threshold value and the drug can be determined to exhibit an acceptable CYP induction potential when the quantitated value does not exceed the threshold value. Isolated peptides are also provided.