ENHANCED ENGINEERED NATIVE PROMOTERS AND USES THEREOF
摘要
We describe methods and DNA constructs / engineered mammalian promoters to enhance native promoter activity while retaining inherent regulation by inserting multi-copy response elements (REs) into non-adjacent locations. Multiple copies of REs are clustered in a group forming a transcription factor response element segment. The segment is at least duplicated in tandem upstream of the ATG start codon. Spacers of 0.2-0.7 kilo base pairs are introduced between the two segments and smaller spacers of about between 9-15 bp are introduced between the copies of REs within a segment.
申请公布号
WO2010141924(A3)
申请公布日期
2011.04.21
申请号
WO2010US37551
申请日期
2010.06.05
申请人
THE GENERAL HOSPITAL CORPORATION;RHOADS, DAVID, B.;HUANG, JIANMIN;LEVITSKY, LYNNE, L.
发明人
RHOADS, DAVID, B.;HUANG, JIANMIN;LEVITSKY, LYNNE, L.