PHOTOBIOCATALYST FOR PRODUCING REDUCED FORMS OF NICOTINAMIDE COENZYMES NADH OR NADPH AND PHOTOCATALYTIC METHOD FOR PRODUCING NADH OR NADPH

摘要

FIELD: medicine. ^ SUBSTANCE: offered is a photobiocatalyst for producing reduced forms of nicotinamide coenzymes NADH or NADPH. The photobiocatalyst represents ferredoxin-NADP-reductase immobilised on a surface of nanopatterned mesoporous titanium dioxide TiO2 film in amount 0.8-1.24 nmole in 1 cm2 of the film surface. The film is prepared of nanocrystalline titanium dioxide of size 15-25 nm and specific surface 50-100 m2/g on a glass substrate or on a glass substrate with a an electrically conductive composition. Also, offered is a photocatalytic method for producing the reduced forms of nicotinamide coenzymes NADH or NADPH with using the photobiocatalyst of ferredoxin-NADP-reductase immobilised on the surface of nanopatterned mesoporous titanium dioxide TiO2 film. The photobiocatalyst allows high speed and specific NADH or NADPH production of NAD+ or NADP+ under exposure to light. ^ EFFECT: method for producing reduced forms of nicotinamide coenzymes NADH or NADPH with using the prepared photobiocatalyst provides easy reaction product separation from an enzyme and a semiconductor, and multiple use of the photobiocatalyst. ^ 4 cl, 5 dwg, 4 ex