ANALYTIC METHOD OFγ-AMINO BUTYRIC ACID FROM GERMINATED RICE

摘要

PURPOSE: A method for easily analyzing GABA content from germinated rice through HPLC is provided. CONSTITUTION: A method for analyzing GABA content from geminated rice comprises: a step of adding a primary extraction solvent containing water, methanol, and chloroform to germinated rice containing GABA; a step of centrifuging to separate a first supernatant and precipitate; a step of adding a secondary extraction solvent to the precipitate and centrifuging to obtain a second supernatant; a step of mixing first and second supernatant and centrifuging to obtain a third supernatant; a step of removing the organic solvent contained in the third supernatant and freeze-drying; a step of dissolving the freeze-dried product into water and filtering; a step of making GABA in a form of 6-aminoquinoline; and a step of analyzing and comparing the liquid from the GABA of 6-aminoquinoline form and standard GABA through HPLC.