METHOD FOR PRODUCING ANTIGEN PREPARATION OF L-BRUCELLAS

摘要

FIELD: medicine. ^ SUBSTANCE: invention refers to medicine and veterinary science, namely to microbiology and immunology. The method involves cultivation of Brucella abortus I-206 L-strain on a dense nutrient medium for L-brucellas at 37C for 3-5 days. Thereafter, a microbial mass is washed of with buffered 0.9% normal saline of pH 7.20.2 and inactivated by adding 2.5% formalin. A bacterial suspension is kept for one day at 37C with specific sterility being controlled. The bacterial suspension is reduced to concentration 4.51010-510 m.c. in ml with buffered 0.9% normal saline (pH 7.20.2) and thermally treated on a water bath at 100C for 45-50 minutes. The suspension is centrifuged at 7000 rpm for 50-60 minutes; the supernatant is separated and frozen-dried. The method allows producing the preparation exhibiting high specificity and activity, available for preparing based immunobiological preparations and a test-system for human and animal blood serum examination for L-brucella antibodies. ^ EFFECT: method is technological, accessible, does not require using expensive devices and equipment. ^ 2 ex