发明名称 |
Method for linear mRNA amplification |
摘要 |
Methods for linearly amplifying mRNA to produce antisense RNA are provided. In the subject methods, mRNA is converted to double-stranded cDNA using a promoter-primer having a poly-dT primer site linked to a promoter sequence so that the resulting double-stranded cDNA is recognized by an RNA polymerase. The resultant double-stranded cDNA is then transcribed into antisense RNA in the presence of a reverse transcriptase that is rendered incapable of RNA-dependent DNA polymerase activity during this transcription step. The subject methods find use a variety of different applications in which the preparation of linearly amplified amounts of antisense RNA is desired. Also provided are kits for practicing the subject methods.
|
申请公布号 |
US7927838(B2) |
申请公布日期 |
2011.04.19 |
申请号 |
US20050179813 |
申请日期 |
2005.07.11 |
申请人 |
AGILENT TECHNOLOGIES, INC. |
发明人 |
SHANNON KAREN W. |
分类号 |
C12Q1/68;C07H21/04;C12N15/10;C12P19/34 |
主分类号 |
C12Q1/68 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|