发明名称 |
MUTANT NANOARCHAEUM EQUITANS A523R DNA POLYMERASE AND ITS USE |
摘要 |
Disclosed herein is a mutant Neq A523R DNA polymerase consisting of an amino acid sequence of SEQ ID NO: 2 wherein alanine at amino acid position 523 in a Nanoarchaeum equitans DNA polymerase (Neq DNA polymerase) consisting of an amino acid sequence of SEQ ID NO: 1 has been substituted with arginine by site-directed mutagenesis. Also disclosed are a gene consisting of a nucleotide sequence encoding the mutant Neq A523R DNA polymerase, a recombinant vector comprising the gene, and a transformant transformed with the vector. In addition, disclosed are a PCR kit comprising the mutant Neq A523R DNA polymerase having excellent performance compared to the wild-type Neq DNA polymerase, and a method for preparing the Neq A523R DNA polymerase.
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申请公布号 |
US2011086387(A1) |
申请公布日期 |
2011.04.14 |
申请号 |
US20100686912 |
申请日期 |
2010.01.13 |
申请人 |
KWON SUK TAE;SONG JAE GEUN;CHO SUNG SUK |
发明人 |
KWON SUK TAE;SONG JAE GEUN;CHO SUNG SUK |
分类号 |
C12P21/06;C07H21/04;C12N1/21;C12N9/12;C12N15/63;C12P19/34 |
主分类号 |
C12P21/06 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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